Analysis of the polyhedrin gene promoter of the autographa californica nuclear polyhedrosis virus 0 nerc institute of virology, mansfield road, oxford ox1 3sr, uk the polyhedrin gene promoter of the autographa californica nuclear polyhedrosis virus was analysed with respect to which sequences are required upstream of the mrna transcription. Lymantria dispar multicapsid nuclear polyhedrosis virus. Polyhedrosis,that is jaundice, is viral infectious, congenital disease of silkworm. The promoter of the late p10 gene in the insect nuclear. The latvian isolate of malacosoma neustria nuclear polyhedrosis virus mn npv is a active and selective agent for the control of the european tent caterpillar. The influence of host plant on the susceptibility of the fall armyworm faw, spodoptera frugiperda j. Pemanfaatan spodoptera litura nuclear polyhedrosis virus. Formulation of a nucleopolyhedrovirus with boric acid for. Cytoplasmic polyhedrosis viruses cpvs are occluded doublestranded rna viruses with a multipartite genome that replicate and form large ca 0. The complete dna sequence of autographa californica. Nuclear polyhedrosis virus an overview sciencedirect topics. Cannibalism was observed commonly even when food was not limiting, but occurred more frequently at low. Pdf detection and analysis of autographa californica. The tolerance of silkworms to bmnpv is controlled by polygenes.
We have generated defective genomes of autographa californica nuclear polyhedrosis virus acnpv by serial, undiluted passage in iplbsf21 cell culture in an attempt to identify potential cisacting sequences important for acnpv dna replication. The degree of control of the fall armyworm, spodoptera frugiperda, by a multiple nucleopolyhedrovirus sfmnpv appears to be limited by the quantity of inoculum consumed by the insect and the delivery of the virus to the insect feeding site. Induction of antitumor acquired immunity by baculovirus. The gypsy moth is univoltine and overwinters in the egg stage. Sequence analysis of the bamhi f fragment of the genome of bombyx mona nuclear polyhedrosis virus. Npv nuclear polyhedrosis virus butterfly fun facts. Isolation and characteristics of the dna from nuclear. Insectspecific viruses can be highly effective natural controls of several caterpillar pests.
We investigated the influence of cytochrome c on apoptosis induced by anagrapha syngrapha falcifera multiple nuclear polyhedrosis virus afmnpv. Microscopic observation revealed that infection of sl. Bioassay of soil to which anticarsia gemmatalis nuclear polyhedrosis virus npv had been applied showed that viral activity was reduced during the summer, but much of the virus remained active. Egg masses were hatched and second instar larvae were fed virusinoculated foliage to propagate the virus in vivo. The stench is horrific and the caterpillars turn to dark black liquid. Nuclear polyhedrosis virus for the control of spodoptera exigua larvae on glasshouse crops. In silkworms, nucleopolyhedrosis virus and viral flacherie are common and can cause very serious larval losses. Oliveira1 1embrapacnpms, caixa postal 151, 35701970, sete lagoas, mg.
Only a single attempt to polyhedrosis virus ldnpv and a nosema sp. Application rate trials with a nuclear polyhedrosis virus. The complete nucleotide sequence of the genome of clone 6 of the baculovirus autographa californica nuclear polyhedrosis virus acnpv has been determined. Molecular analysis of an enhancin gene in the lymantria.
Review paper nuclear polyhedrosis virus npv, a potential biopesticide. Chaudhari2 abstract the susceptibility of different stages of spodoptera litura larvae to npv was determined by bioassay. Molecular engineering of the autographa californica nuclear. Baculovirus preparations registered for pest control in various parts of the world are mentioned.
Pdf transovarial transmission of bombyx mori nuclear. Among the viruses of polyhedrosis, the nuclear polyhedrosis virus npvis distinguished by its rather high. Epizootics can occasionally devastate populations of some pests, especially when insect numbers are high. The pathology of the nuclear polyhedrosis virus from spodoptera littoralis against kalotermes flavicollis was confirmed under light microscope. Generally, two different types of membrane are seen, which we categorize as envelopes and transport membranes. Brasil 261 145 application rate trials with a nuclear polyhedrosis virus to control spodoptera frugiperda smith on maize ivan cruz 1, maria l. Nigel venters found this article that is a basic, laymans description of nuclear polyhedrosis, and stated that this article was consistent with his experience raising butterflies for 50 years. The nob including the oryctes rhinoceros virus orv and the heliothis helicoverpa zea virus 1 hzv1 has been removed from this family and are not assigned to any virus family. The nuclear polyhedrosis virus npv which belongs to the sub group baculoviruses is a virus affecting insects, predominantly moths and butterflies. Uji patogenisitas beberapa isolat sl npv spodoptera. Previous studies suggest that the penetration of nucleocapsids induces early actin cables followed by further changes of the actin cytoskeleton which depend on early viral gene expression.
The entomopathogenic viruses like nuclear polyhedrosis virus npv, a member of baculoviruses, can be the potential candidates for better control against abw. The nuclear polyhedrosis virus npv, part of the family of baculoviruses, is a virus affecting. Evaluation of aeriallyapplied nuclear polyhedrosis virus. A baculovirus expression vector derived from the basic.
Electron microscopy of purified virus particles and nucleocapsids demonstrated them to be similar in structure to previously recorded nuclear polyhedrosis viruses. Two strains of a nuclear polyhedrosis virus npv of trichoplusia ni were isolated on the basis of plaque morphology. Isolation, identification, and determination of virulence of a nuclear. Bioassay of nuclear polyhedrosis virus npv and in combination with insecticide on spodoptera litura fab tran thi kieu trang 1 and s. A kondo and s maeda faculty of agriculture, tottori university, japan. Characteristics of the nonoccluded form of a nuclear polyhedrosis. Nuclear polyhedrosis virus as a biological control agent for malacosoma americanum lepidoptera. In lepidopteran insect cells infected with the baculovirus autographa californica nuclear polyhedrosis virus acnpv, two major late viral gene products are expressed. The polyhedrin gene promoter of the autographa californica nuclear polyhedrosis virus was analysed with respect to which sequences are required upstream of the mrna transcription initiation cap site for efficient promoter activity.
Defective interfering particles dips were generated upon continuous production of autographa californica nuclear polyhedrosis virus acnpv in bioreactors. New formulations of the heliothis nuclear polyhedrosis virus were made by incorporating the pathogen with uv light screening agents in a capsule bound together with digestible, waterinsoluble polymers. Nuclear polyhedrosis virus an overview sciencedirect. Histopathology of termite kalotermes flavicollis fabr. Larvae emerge from eggs coincident with budbreak in the spring and mature through five or,six larval instars over a period of about 8 weeks. Host range expansion by recombination of the baculoviruses bombyx mori nuclear polyhedrosis virus and autographa californica nuclear polyhedrosis virus. This configuration mimicked the serial undiluted passaging of virus, which is known to. Nuclear polyhedrosis virus, biopesticide, baculovirus, characterizati on. The baculovirus autographa californica multiple nuclear polyhedrosis virus acmnpv has been studied as a gene therapy vector.
Eubaculovirinae includes two genera, nuclear polyhedrosis virus npv and granulosis virus gv. Bombyx mori nuclear polyhedrosis virus bmnpv is an important pathogen of silkworms. The formulation of the virus with phagostimulants andor viral synergists, such as boric acid, may help overcome this problem. Nonoccluded virions of a nuclear polyhedrosis virus of the alfalfa looper, autographa californica, found in the medium of cell cultures of infected.
Nuclear polyhedrosis virus international butterfly. Nuclear polyhedrosis virus journal of virology asm. Nuclear polyhedrosis virus as a biological control agent for. Nuclear polyhedrosis virus as a biological control agent. The genome of the nuclear polyhedrosis virus which infects the larvae of the tussock moth, the lepidopteran, hemerocampa pseudotsugata, was isolated, identified as deoxyribonucleic acid, and characterized in terms of its physical and biological properties.
Fulllength cdna coding for the human transcriptional intermediary factor 1 gamma fused to a decahistidine purification tag. This portugal demonstrated that the interaction of two early biological control effort by speare and colley. Sections of infected termites showed a comprehensive viral infection. Generation and analysis of defective genomes of autographa californica nuclear polyhedrosis virus. Nuclei of cells of the foregut, cardiac valve, pyloric valve, hindgut, gut musculature, fat body, nerve tissue, muscle, oviduct, pedicel of he ovariole, calyx.
Bioassays demonstrated that two of these, one made with titanium dioxide and the other with carbon black, were remarkably tolerant of the uv. Loss of viability of a nuclear polyhedrosis virus npv of spodoptera littoralis was shown to occur on cotton leaves but not on cabbage leaves or on semisynthetic insect diet. Membranes are intimately involved in the lifecycle of nuclear polyhedrosis viruses npv, and the related bellett, 1969 granulosis viruses gv. Nuclear polyhedrosis virus, biopesticide, baculovirus, characterization. Here, we demonstrated that acmnpv induces antitumor acquired immunity. In this paper we report the results of studies on the identification and host range of this isolate, and its. Viral activity in the upper layer of soil was generally greater following tillage, and the level of virus activity on plants was related to the concentration of npv applied. Bionomi npv klasifikasi dan deskripsi npv npv merupakan salah satu anggota genus baculovirus, famili baculoviridaeyang memiliki dua genus, yaitu nucleo polyhedrosis virus npv dan granulovirus gv murphy et al.
Effect of host plant on the susceptibility of spodoptera. Lewis evaluation of aeriallyapplied nuclear polyhedrosis virus for suppression of the gypsy moth, lymantria. The former represent those membranes which closely surround mature, occluded, nucleocapsids and which are also. The basic protein of autographa californica nuclear polyhedrosis virus acmnpv is associated with virus dna in virion nucleocapsids and is produced in infected cells during the late phase of gene expression. Identification of the early actinrearrangementinducing. Bmnpv, nucleopolyhedrosis virus, a doublestranded dna baculovirus that infects silkworms and is a serious problem in sericulture, has been engineered as an expression vector to produce exogenous products such as interferon for commercial use and has some applications in the pharmaceutical industry. A bmnpv induced the formation of autophagic bodies by transmission electron microscopy tem analysis. Pdf inactivation of a nuclear polyhedrosis virus on. Comparative bioefficacy of nuclear polyhedrosis virus. At subsequent evaluation dates 9,11 and 14 days after. An aqueous suspension of the nuclear polyhedrosis virus of lymantria dispar l. Our analysis suggests that the virus encodes some 154 methionineinitiated. The inactivation was rapid, occurred in the dark and required contact. Smith, to a nuclear polyhedrosis virus npv was investigated.
Nuclear polyhedrosis virus npv, a potential biopesticide. Acmnpv infection of spodoptera frugiperda sf9 insect cells. The nuclear polyhedrosis virus npv, part of the family of baculoviruses, is a virus affecting insects, predominantly moths and butterflies. Cannibalism was observed commonly even when food was not limiting, but occurred more frequently at low food quantities and. Uji patogenisitas beberapa isolat sl npv spodoptera litura nuclear polyhedrosis virus terhadap tingkat mortalitas ulat grayak spodoptera litura f. These results suggest that acmnpv has the potential to be an efficient virus or tumor therapy agent which induces innate and acquired immunity. L applied to small pellets of artificial diet or isolated surfaces of. In response to autographa californica multicapsid nuclear polyhedrosis virus acmnpv infection, a sequential rearrangement of the actin cytoskeleton occurs. Characterization of a nuclear polyhedrosis virus isolated. Different strains of naturally occurring nuclear polyhedrosis virus npv and granulosis virus are present at low levels in many insect populations. Chances are they have npv, nuclear polyhedrosis virus.
Despite extensive studies in recent decades, the interaction between. Host range expansion by recombination of the baculoviruses. Experiments were carried out to investigate the incidence of cannibalism throughout the larval development of the noctuid moth spodoptera frugiperda, and to examine the risk of infection from consuming conspecifics infected with a nuclear polyhedrosis virus sfnpv 2. Analysis of the polyhedrin gene promoter of the autographa. Melt, wilt, and black death are a few of the other names that are used for this virus. All of the tested additives gave good wettability of dispersible dry formulations jankevica, zarins, 1997 as well as promoted adhesion to the plants. For the expression of recombinant proteins and biological control of insect pests they are extensively studied 6. Bmnpv bombyx mori nuclear polyhedrosis virus causes nuclear polyhedrosis in silkworms. Seven days after inoculation, the median lethal concentration lc 50 was 1. Spodoptera litura nuclear polyhedrosis virus slnpv berpotensi dikembangkan untuk mengendalikan s. Movement of a nuclear polyhedrosis virus from soil to. The larvae then melt or disintegrate, falling onto the foliage below, where they infect more larvae.
244 1300 1441 1319 639 1179 1250 515 287 274 1389 735 1251 1031 264 313 1672 318 1308 1088 993 1619 1494 81 1451 516 1053 1 253 693 877 239